Brazilian scientific production in Biochemistry increased in recent years, and the state of Rio Grande do Sul (RS) presented significant relevance in this context. The objective of this study is to characterize the profile of researchers in Biochemistry of public and private universities of RS and to quantify and analyze their scientific production (scientific papers; Ms and PhD theses). We observed that the majority of the researchers in Biochemistry in public and private institutions have a PhD degree. However, various researchers do not develop research activities at the institutions where they are working.